The clinical significance of antibody to vascular endothelial cells after renal transplantation

Clin Transplant. 2002;16 Suppl 8:51-7. doi: 10.1034/j.1399-0012.16.s8.10.x.

Abstract

Vascular endothelial cells (ECs) are considered to be a primary target for injury in allograft rejection. However, the relationship between serum antibody activity to ECs and rejection episodes has not been examined extensively in renal transplantation. Twenty-two renal transplant recipients were included in this study. Serum antibody activity to vascular endothelial cells (AECA) was measured using a cellular enzyme-linked immunosorbent assay (ELISA) in which human umbilical vein endothelial cells (HUVEC) and human glomerular endothelial cells (HGEC) were preincubated with TNF-alpha used as target cells. Serum samples were obtained just before transplantation and once a week during the immediate 1-3-month post-transplantation period. There was a significant correlation between the presence of AECA against HGEC and rejection episodes (P < 0.05). Patients with multi-episodes of rejection showed significantly higher frequencies of AECA than patients with mono-episodic rejection (P < 0.0005). It should be noted that patients suffering from multi-episodes of rejection revealed higher AECA titres before transplantation. These findings imply that the HGEC-ELISA could be used as a prospective, informative test to identify patients with a higher risk of acute rejection in renal transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Autoantibodies / blood*
  • Child
  • Cyclosporine / therapeutic use
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Graft Rejection / immunology
  • Humans
  • Immunosuppressive Agents
  • Kidney Transplantation*
  • Male
  • Middle Aged
  • Tacrolimus / therapeutic use

Substances

  • Autoantibodies
  • Immunosuppressive Agents
  • Cyclosporine
  • Tacrolimus