Use of a tyrosine hydroxylase mutant enzyme with reduced metal affinity allows detection of activity with cobalt in place of iron

Arch Biochem Biophys. 2002 Dec 15;408(2):305-7. doi: 10.1016/s0003-9861(02)00568-4.
No abstract available

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • Biochemistry / methods*
  • Cobalt / analysis
  • Cobalt / metabolism*
  • Iron / metabolism*
  • Mutation*
  • Pterins / metabolism
  • Spectrophotometry, Atomic
  • Tyrosine 3-Monooxygenase / genetics*
  • Tyrosine 3-Monooxygenase / metabolism*

Substances

  • Pterins
  • tetrahydropterin
  • Cobalt
  • Iron
  • Tyrosine 3-Monooxygenase