Electron tomography: towards visualizing the molecular organization of the cytoplasm

Curr Opin Struct Biol. 2002 Oct;12(5):679-84. doi: 10.1016/s0959-440x(02)00378-0.


Electron tomography of thin sections of plastic-embedded biological material provides valuable insights into the organellar organization of cells. With the advent of automated data-acquisition procedures, it has become possible to study radiation-sensitive specimens, such as frozen-hydrated organelles and whole cells. This opens up exciting perspectives for visualizing the molecular organization of the cytoplasm.

Publication types

  • Review

MeSH terms

  • Animals
  • Cytoplasm / ultrastructure*
  • Electrons*
  • Freezing
  • Image Processing, Computer-Assisted
  • Islets of Langerhans / ultrastructure
  • Muscle, Skeletal / ultrastructure
  • Organelles / ultrastructure*
  • Tomography / methods*