Comparison of automated cellular imaging system and manual microscopy for immunohistochemically stained cryostat sections of lung cancer specimens applying p53, ki-67 and p120

Oncol Rep. 2003 Jan-Feb;10(1):15-20.


Seventy-one frozen lung tissue specimens from 52 patients suffering from non-small cell lung cancer (NSCLC) were analysed in this study. Cryostat sections were stained with monoclonal antibodies against p53, ki-67 and p120, all of which are of prognostic value in NSCLC. Slides were evaluated by standard manual microscopy (MM) and using a new Automated Cellular Imaging System (ACIS). The results obtained with ACIS correlated significantly with MM examination (p<0.001). However, ACIS showed a higher sensitivity, especially for specimens with a low infiltration volume. In 15 (6.8%) MM negative cases singular positive cells were identified with ACIS. In cases with a high infiltration volume subjective (MM) quantitation tended to overestimate the number of infiltrating cells. ACIS guaranteed a high reproducibility of data. We conclude that ACIS-assisted analysis is a valid means of investigating the p53, ki-67 and p120 antibody expression in cryostat sections of lung cancer specimens. ACIS can complement conventional manual microscopy due to its higher accuracy, sensitivity and better reproducibility of data.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / pathology
  • Automation
  • Biomarkers, Tumor / metabolism
  • Carcinoma, Non-Small-Cell Lung / chemistry
  • Carcinoma, Non-Small-Cell Lung / metabolism*
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Cohort Studies
  • Diagnostic Imaging
  • Humans
  • Image Processing, Computer-Assisted
  • Immunoenzyme Techniques
  • Ki-67 Antigen / metabolism*
  • Lung Neoplasms / chemistry
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / pathology
  • Microscopy
  • Nuclear Proteins / metabolism*
  • Prognosis
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tumor Suppressor Protein p53 / metabolism*
  • tRNA Methyltransferases


  • Biomarkers, Tumor
  • Ki-67 Antigen
  • Nuclear Proteins
  • Tumor Suppressor Protein p53
  • NOP2 protein, human
  • tRNA Methyltransferases