Differentiation of human gamma-delta T cells towards distinct memory phenotypes

Cell Immunol. Jul-Aug 2002;218(1-2):1-6. doi: 10.1016/s0008-8749(02)00519-1.


Vgamma9/Vdelta2 T cells comprise a small population of peripheral T cells responding towards the low molecular weight antigen, (E)-4-hydroxy-3-methyl-but-2-enyl-pyrophosphate (HMB-PP). HMB-PP-stimulated Vgamma9/Vdelta2 T cells proliferated, expressed CCL5/RANTES, and upregulated markers like CD16, CD25, CD69, and CD94, in the presence of either IL-15 or IL-21. Vgamma9/Vdelta2 T cells grown in the presence of IL-15 differentiated into an effector/memory population characterized by production of TNF-alpha, expression of CD45RO and CCR5, and lack of CD62L, CD81, and CCR7. In contrast, Vgamma9/Vdelta2 T cells grown with IL-21 differentiated into putative central memory CD45RO(+) T cells that did not produce TNF-alpha, IFN-gamma, or IL-4, and maintained expression of CD62L, CD81, and CCR7.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / biosynthesis*
  • Cell Differentiation / drug effects
  • Cells, Cultured / cytology
  • Cells, Cultured / immunology
  • Chemokine CCL5 / biosynthesis
  • Diphosphates / immunology
  • Flow Cytometry
  • Gene Expression Regulation / immunology
  • Humans
  • Immunologic Memory / immunology*
  • Inflammation Mediators / metabolism
  • Interleukin-15 / pharmacology*
  • Interleukins / pharmacology*
  • Lectins, C-Type / biosynthesis
  • NK Cell Lectin-Like Receptor Subfamily D
  • Receptors, Antigen, T-Cell, gamma-delta / immunology*
  • Receptors, IgG / biosynthesis
  • T-Lymphocyte Subsets / cytology
  • T-Lymphocyte Subsets / immunology*


  • 4-hydroxy-3-methylbut-2-enyl pyrophosphate
  • Antigens, CD
  • Chemokine CCL5
  • Diphosphates
  • Inflammation Mediators
  • Interleukin-15
  • Interleukins
  • KLRD1 protein, human
  • Lectins, C-Type
  • NK Cell Lectin-Like Receptor Subfamily D
  • Receptors, Antigen, T-Cell, gamma-delta
  • Receptors, IgG
  • interleukin-21