Sinusoidal efflux of taurocholate correlates with the hepatic expression level of Mrp3

Biochem Biophys Res Commun. 2002 Dec 20;299(5):681-7. doi: 10.1016/s0006-291x(02)02723-7.


Multidrug resistance-associated protein 3 (Mrp3/ABCC3), which can mediate the cellular extrusion of bile acids, is induced on the hepatic sinusoidal membrane of Mrp2/ABCC2-deficient rats (Eisai hyperbilirubinemic rats; EHBRs) and phenobarbital-treated Sprague-Dawley rats. In the present study, the correlation between the sinusoidal efflux clearance (PS(eff)) of [3H]taurocholate (TC) and the hepatic expression of Mrp3 was investigated using perfused liver from these rats. A significant correlation was observed between the PS(eff) and the hepatic expression level of Mrp3, suggesting a contribution by Mrp3 to the sinusoidal efflux of TC. The results of the kinetic analysis also suggested that other transporter(s) on the sinusoidal plasma membrane may participate in the efflux of TC under physiological conditions. The contribution of Mrp3 to the sinusoidal efflux of TC in EHBRs and phenobarbital (80 and 40 mg/kg)-treated rats was revealed to be 58%, 48%, and 31%, respectively.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Cell Membrane / metabolism*
  • Cell Polarity
  • Kinetics
  • Liver / drug effects
  • Liver / metabolism*
  • Male
  • Multidrug Resistance-Associated Proteins / metabolism*
  • Perfusion
  • Phenobarbital / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Taurocholic Acid / metabolism*


  • Multidrug Resistance-Associated Proteins
  • multidrug resistance-associated protein 3
  • Taurocholic Acid
  • Phenobarbital