Rab11-FIP4 interacts with Rab11 in a GTP-dependent manner and its overexpression condenses the Rab11 positive compartment in HeLa cells

Biochem Biophys Res Commun. 2002 Dec 20;299(5):770-9. doi: 10.1016/s0006-291x(02)02720-1.


We have recently identified Rab11-FIP4 as the sixth member of the Rab11-FIP family of Rab11 interacting proteins. Here, we demonstrate that Rab11-FIP4 interacts with Rab11 in a GTP-dependent manner and that its C-terminal region allows the protein to self-interact and interact with pp75/Rip11, Rab11-FIP2, and Rab11-FIP3. However, Rab11-FIP4 does not appear to interact directly with Rab coupling protein (RCP). We investigated the subcellular localisation of Rab11-FIP4 in HeLa cells and show that it colocalises extensively with transferrin and with Rab11. Furthermore, when overexpressed, it causes a condensation of the Rab11 compartment in the perinuclear region. We demonstrate that the carboxy-terminal region of Rab11-FIP4 (Rab11-FIP4(C-ter)) is necessary and sufficient for its endosomal membrane association. Expression of Rab11-FIP4(C-ter) causes a dispersal of the Rab11 compartment towards the cell periphery and does not inhibit transferrin recycling in HeLa cells. It is likely that Rab11-FIP4 serves as a Rab11 effector in a Rab11 mediated function other than transferrin recycling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Compartmentation
  • Endosomes / chemistry*
  • Guanosine Triphosphate / metabolism*
  • HeLa Cells
  • Humans
  • Intracellular Membranes / chemistry
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Protein Transport
  • Sequence Deletion
  • Transfection
  • Transferrin / metabolism
  • rab GTP-Binding Proteins / analysis*
  • rab GTP-Binding Proteins / metabolism*


  • Carrier Proteins
  • Membrane Proteins
  • RAB11FIP4 protein, human
  • Transferrin
  • Guanosine Triphosphate
  • rab11 protein
  • rab GTP-Binding Proteins