Molecular Identification of the First Insect Ecdysis Triggering Hormone Receptors

Biochem Biophys Res Commun. 2002 Dec 20;299(5):924-31. doi: 10.1016/s0006-291x(02)02798-5.


The Drosophila Genome Project website ( contains an annotated gene sequence (CG5911), coding for a G protein-coupled receptor. We cloned the cDNA corresponding to this sequence and found that the gene has not been correctly predicted. The corrected gene CG5911 has five introns and six exons (1-6). Alternative splicing yields two cDNAs called A (containing exons 1-5) and B (containing exons 1-4, 6). We expressed these splicing variants in Chinese hamster ovary cells and found that the corrected CG5911-A and -B cDNAs coded for two different G protein-coupled receptors that could be activated by low concentrations of Drosophila ecdysis triggering hormones-1 and -2. Ecdysis (cuticle shedding) is an important behaviour, allowing growth and metamorphosis in insects and other arthropods. Our paper is the first report on the molecular identification of ecdysis triggering hormone receptors from insects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells
  • Cricetinae
  • Drosophila / embryology
  • Drosophila / growth & development*
  • Drosophila / metabolism
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Drosophila Proteins / physiology
  • Insect Hormones / pharmacology
  • Molecular Sequence Data
  • Molting*
  • RNA, Messenger / biosynthesis
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / physiology
  • Receptors, G-Protein-Coupled*
  • Receptors, Neuropeptide / genetics*
  • Receptors, Neuropeptide / metabolism
  • Sequence Homology, Amino Acid


  • Drosophila Proteins
  • ETHR protein, Drosophila
  • Insect Hormones
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Receptors, Neuropeptide
  • ecdysis-triggering hormone, Drosophila

Associated data

  • GENBANK/AF505863
  • GENBANK/AF505864