Induction of astrocytic cyclooxygenase-2 in epileptic patients with hippocampal sclerosis

Neurochem Int. 2003 Mar;42(4):299-303. doi: 10.1016/s0197-0186(02)00101-8.


Induction of cyclooxygenase-2 (COX-2) has been described in a wide range of neurological diseases including animal models of epilepsy. The present study was undertaken to assess COX-2 expression in hippocampal biopsies from patients with therapy-refractive temporal lobe epilepsy (TLE). For this purpose, hippocampal CA1 subfield was dissected from epileptic patients with (n=5) or without (n=2) hippocampal sclerosis (HS). COX-2 expression was investigated using immunohistochemistry and semi-quantitative RT-PCR. COX-2 immunoreactivity in TLE patient material in the absence of HS was restricted to a few neurons of the hippocampus. In the presence of HS, on the other hand, a significant induction of astrocytic COX-2 immunoreactivity associated with a concomitant increase in the steady-state level of COX-2 mRNA was observed in the CA1 subfield. These findings suggest that induction of astrocytic COX-2 is implicated in the pathogenesis of HS in TLE and is consistent with the previous findings of increased concentrations of prostaglandins in the cerebrospinal fluid of these patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Astrocytes / enzymology*
  • Cyclooxygenase 2
  • Epilepsy, Temporal Lobe / enzymology*
  • Epilepsy, Temporal Lobe / pathology
  • Female
  • Hippocampus / pathology*
  • Humans
  • Immunohistochemistry
  • Isoenzymes / biosynthesis*
  • Male
  • Membrane Proteins
  • Prostaglandin-Endoperoxide Synthases / biosynthesis*
  • RNA / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sclerosis / enzymology*
  • Sclerosis / pathology


  • Isoenzymes
  • Membrane Proteins
  • RNA
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases