Polyribosome loading of spinach mRNAs for photosystem I subunits is controlled by photosynthetic electron transport

Plant J. 2002 Dec;32(5):631-9. doi: 10.1046/j.1365-313x.2002.01452.x.

Abstract

In light-, but not in dark-grown spinach seedlings, the mRNAs for the nuclear-encoded photosystem I subunits D, F and L are associated with polyribosomes and this association is prevented by the application of 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea (DCMU), an inhibitor of the photosynthetic electron transport. To identify the cis-elements which are responsible for this regulation, we generated a series of chimeric PsaD constructs and tested them in transgenic tobacco. The spinach PsaD 5'-untranslated region is sufficient to confer light- and photosynthesis-dependent polyribosome association onto the uidA reporter gene, while the tobacco PsaD 5'-untranslated region directs constitutive polyribosome association. These results are discussed with regard to signals from photosynthetic electron flow which control processes in the cytoplasm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Darkness
  • Electron Transport
  • Light
  • Molecular Sequence Data
  • Photosynthesis*
  • Photosystem I Protein Complex / genetics*
  • Plants, Genetically Modified
  • Polyribosomes / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • Spinacia oleracea / genetics*
  • Spinacia oleracea / metabolism*
  • Time Factors
  • Tobacco / genetics
  • Tobacco / metabolism

Substances

  • Photosystem I Protein Complex
  • RNA, Messenger