Arabidopsis thaliana root non-selective cation channels mediate calcium uptake and are involved in growth

Plant J. 2002 Dec;32(5):799-808. doi: 10.1046/j.1365-313x.2002.01467.x.


Calcium is a critical structural and regulatory nutrient in plants. However, mechanisms of its uptake by root cells are poorly understood. We have found that Ca2+ influx in Arabidopsis root epidermal protoplasts is mediated by voltage-independent rapidly activating Ca2+-permeable non-selective cation channels (NSCCs). NSCCs showed the following permeability (P) sequence: PCa (1.00) = PBa (0.93) > PZn (0.51), PCa/PNa = 0.19, PCa/PK = 0.14. They were inhibited by quinine, Gd3+, La3+ and the His modifier diethylpyrocarbonate, but not by the Ca2+ or K+ channel antagonists, verapamil and tetraethylammonium (TEA+). Single channel conductance measured in 20 mm external Ca2+ was 5.9 pS. Calcium-permeable NSCCs co-existed with hyperpolarisation-activated Ca2+ channels (HACCs), which activated 40-60 min after forming the whole-cell configuration. HACCs activated at voltages <-130 to -150 mV, showed slow activation kinetics and were regulated by cytosolic Ca2+ ([Ca2+]cyt). Using aequorin-expressing plants, a linear relationship between membrane potential (Vm) and resting [Ca2+]cyt was observed, indicating the involvement of NSCCs. Intact root 45Ca2+ influx was reduced by Gd3+ (NSCC blocker) but was verapamil and TEA+ insensitive. In the root elongation zone, both root net Ca2+ influx (measured by Ca2+-selective vibrating microelectrode) and NSCC activity were increased compared to the mature epidermis, suggesting the involvement of NSCC in growth. A Ca2+ acquisition system based on NSCC and HACC co-existence is proposed. In mature epidermal cells, NSCC-mediated Ca2+ influx dominates whereas in specialised root cells (root hairs and elongation zone cells) where elevated [Ca2+]cyt activates HACCs, HACC-mediated Ca2+ influx predominates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiporters / metabolism*
  • Arabidopsis / cytology
  • Arabidopsis / drug effects
  • Arabidopsis / growth & development*
  • Arabidopsis / metabolism*
  • Biological Transport, Active / drug effects
  • Calcium / metabolism*
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Cell Membrane Permeability / drug effects
  • Gadolinium / pharmacology
  • Ion Channel Gating
  • Ion Transport / drug effects
  • Membrane Potentials / drug effects
  • Plant Epidermis / cytology
  • Plant Epidermis / drug effects
  • Plant Epidermis / metabolism
  • Plant Roots / cytology
  • Plant Roots / drug effects
  • Plant Roots / metabolism*
  • Potassium / metabolism
  • Protoplasts / cytology
  • Protoplasts / drug effects
  • Protoplasts / metabolism
  • Verapamil / pharmacology


  • Antiporters
  • Gadolinium
  • Verapamil
  • Potassium
  • Calcium