Juice and phenolic fractions of the berry Aristotelia chilensis inhibit LDL oxidation in vitro and protect human endothelial cells against oxidative stress

J Agric Food Chem. 2002 Dec 18;50(26):7542-7. doi: 10.1021/jf025797n.


Oxidative modification of low-density lipoprotein (LDL) particles is a key event in the development of atherosclerosis. Oxidized LDL induces oxidative stress and modifies gene expression in endothelial cells. Berries constitute a rich dietary source of phenolic antioxidants. We found that the endemic Chilean berry Aristotelia chilensis (ach) has higher phenol content and scores better for total radical-trapping potential and total antioxidant reactivity in in vitro antioxidant capacity tests, when compared to different commercial berries. The juice of ach is also effective in inhibiting copper-induced LDL oxidation. In human endothelial cell cultures, the addition of ach juice significantly protects from hydrogen peroxide-induced intracellular oxidative stress and is dose-dependent. The aqueous, anthocyanin-rich fraction of ach juice accounts for most of ach's antioxidant properties. These results show that ach is a rich source of phenolics with high antioxidant capacity and suggest that it may have antiatherogenic properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Cells, Cultured
  • Copper / chemistry
  • Endothelium, Vascular / drug effects*
  • Fruit / chemistry*
  • Humans
  • Hydrogen Peroxide / pharmacology
  • Lipid Peroxidation / drug effects
  • Lipoproteins, LDL / chemistry*
  • Magnoliopsida / chemistry*
  • Oxidation-Reduction
  • Oxidative Stress / drug effects
  • Phenols / analysis
  • Phenols / pharmacology*
  • Plant Extracts / chemistry
  • Plant Structures / chemistry
  • Umbilical Veins
  • Wine / analysis


  • Antioxidants
  • Lipoproteins, LDL
  • Phenols
  • Plant Extracts
  • Copper
  • Hydrogen Peroxide