Evidence for packaging of rep-cap sequences into adeno-associated virus (AAV) type 2 capsids in the absence of inverted terminal repeats: a model for generation of rep-positive AAV particles

J Virol. 2003 Jan;77(1):776-81. doi: 10.1128/jvi.77.1.776-781.2003.

Abstract

We previously reported that a 350-bp region of the adeno-associated virus (AAV) type 2 rep gene contains a cis-acting element responsible for the Rep-dependent replication of a transiently transfected rep-cap plasmid. In this study, we further report that replicated rep-cap sequences can be packaged into AAV capsids in the absence of the inverted terminal repeats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid / chemistry*
  • DNA-Binding Proteins / genetics*
  • Dependovirus / physiology*
  • Plasmids
  • Terminal Repeat Sequences*
  • Viral Proteins / genetics*
  • Virion / physiology*
  • Virus Assembly*

Substances

  • DNA-Binding Proteins
  • Viral Proteins
  • rep proteins, Adeno-associated virus 2