Hodgkin's lymphoma cell lines are characterized by frequent aberrations on chromosomes 2p and 9p including REL and JAK2

Int J Cancer. 2003 Feb 10;103(4):489-95. doi: 10.1002/ijc.10845.


Four Hodgkin's lymphoma cell lines (KM-H2, HDLM-2, L428, L1236) were analyzed for cytogenetic aberrations, applying multiplex fluorescence in situ hybridization, chromosome banding and comparative genomic hybridization. Each line was characterized by a highly heterogeneous pattern of karyotypic changes with a large spectrum of different translocated chromosomes (range 22-57). A recurrent finding in all cell lines was the presence of chromosomal rearrangements of the short arm of chromosome 2 involving the REL oncogene locus. Furthermore, multiple translocated copies of telomeric chromosomal segments were frequently detected. This resulted in a copy number increase of putative oncogenes, e.g., JAK2 (9p24) in 3 cell lines, FGFR3 (4p16) and CCND2 (12p13) in 2 cell lines as well as MYC (8q24) in 1 cell line. Our data confirm previous cytogenetic results from primary Hodgkin's tumors suggesting an important pathogenic role of REL and JAK2 in this disease. In addition, they provide evidence for a novel cytogenetic pathomechanism leading to increased copy numbers of putative oncogenes from terminal chromosomal regions, most probably in the course of chromosomal stabilization by telomeric capture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Aberrations
  • Chromosome Banding
  • Chromosome Painting
  • Chromosomes, Human, Pair 2*
  • Chromosomes, Human, Pair 9*
  • Hodgkin Disease / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Janus Kinase 2
  • Karyotyping
  • Models, Genetic
  • Nucleic Acid Hybridization
  • Protein-Tyrosine Kinases / genetics*
  • Proto-Oncogene Proteins c-rel / genetics*
  • Proto-Oncogene Proteins*
  • Telomere
  • Translocation, Genetic
  • Tumor Cells, Cultured


  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-rel
  • Protein-Tyrosine Kinases
  • JAK2 protein, human
  • Janus Kinase 2