Self- and actin-templated assembly of Mammalian septins

Dev Cell. 2002 Dec;3(6):791-802. doi: 10.1016/s1534-5807(02)00366-0.

Abstract

Septins are polymerizing GTPases required for cytokinesis and cortical organization. The principles by which they are targeted to, and assemble at, specific cell regions are unknown. We show that septins in mammalian cells switch between a linear organization along actin bundles and cytoplasmic rings, approximately 0.6 microm in diameter. A recombinant septin complex self-assembles into rings resembling those in cells. Linear organization along actin bundles was reconstituted by adding an adaptor protein, anillin. Perturbation of septin organization in cells by expression of a septin-interacting fragment of anillin or by septin depletion via siRNA causes loss of actin bundles. We conclude that septins alone self-assemble into rings, that adaptor proteins recruit septins to actin bundles, and that septins help organize these bundles.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Actin Cytoskeleton / metabolism*
  • Adaptor Proteins, Signal Transducing
  • Animals
  • Binding Sites / physiology
  • Blood Proteins / genetics
  • Blood Proteins / metabolism
  • Carrier Proteins*
  • Cell Compartmentation / physiology*
  • Contractile Proteins / metabolism
  • Cytoskeletal Proteins
  • Eukaryotic Cells / cytology
  • Eukaryotic Cells / metabolism*
  • GTP Phosphohydrolase Activators*
  • GTP Phosphohydrolases
  • GTP-Binding Protein Regulators*
  • GTP-Binding Proteins / deficiency*
  • GTP-Binding Proteins / genetics*
  • Green Fluorescent Proteins
  • HeLa Cells
  • Humans
  • Luminescent Proteins
  • Mice
  • Protein Structure, Tertiary / physiology
  • Recombinant Fusion Proteins
  • Septins
  • cdc42 GTP-Binding Protein*

Substances

  • Adaptor Proteins, Signal Transducing
  • Blood Proteins
  • CDC42EP2 protein, human
  • CDC42EP3 protein, human
  • CDC42EP4 protein, human
  • CDC42EP5 protein, human
  • Carrier Proteins
  • Cdc42ep5 protein, mouse
  • Contractile Proteins
  • Cytoskeletal Proteins
  • GTP Phosphohydrolase Activators
  • GTP-Binding Protein Regulators
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • anillin
  • Green Fluorescent Proteins
  • CDC42EP1 protein, human
  • GTP Phosphohydrolases
  • GTP-Binding Proteins
  • SEPTIN6 protein, human
  • Sept6 protein, mouse
  • Septins
  • cdc42 GTP-Binding Protein