Scabrous controls ommatidial rotation in the Drosophila compound eye

Dev Cell. 2002 Dec;3(6):839-50. doi: 10.1016/s1534-5807(02)00362-3.

Abstract

Establishment of planar polarity in the Drosophila compound eye requires precise 90 degrees rotation of the ommatidial clusters during development. We found that the morphogenetic furrow controls the stop of ommatidial rotation at 90 degrees by emitting signals to posterior ommatidial clusters. One such signal, Scabrous, is synthesized in the furrow cells and transported in vesicles to ommatidial row 6-8. Scabrous vesicles are transported through actin-based cellular extensions but not transcytosis. Scabrous functions nonautonomously to control the stop of ommatidial rotation by suppressing nemo activity in the second 45 degrees rotation. We propose that the morphogenetic furrow regulates precise ommatidial rotation by transporting Scabrous and perhaps other factors through actin-based cellular extensions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / drug effects
  • Actin Cytoskeleton / metabolism
  • Animals
  • Body Patterning / physiology*
  • Cell Movement / physiology*
  • Cell Polarity / physiology*
  • Cell Surface Extensions / genetics
  • Cell Surface Extensions / metabolism
  • Cell Surface Extensions / ultrastructure
  • Cytochalasin D / pharmacology
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / cytology
  • Drosophila melanogaster / embryology*
  • Drosophila melanogaster / metabolism
  • Female
  • Gene Expression Regulation, Developmental / physiology
  • Glycoproteins / genetics
  • Glycoproteins / metabolism*
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • Male
  • Microscopy, Fluorescence
  • Microtubules / drug effects
  • Microtubules / metabolism
  • Microtubules / ultrastructure
  • Mitogen-Activated Protein Kinases / genetics
  • Mitogen-Activated Protein Kinases / metabolism
  • Mutation / physiology
  • Photoreceptor Cells, Invertebrate / cytology
  • Photoreceptor Cells, Invertebrate / embryology*
  • Photoreceptor Cells, Invertebrate / metabolism
  • Protein Transport / physiology*
  • Recombinant Fusion Proteins
  • Transport Vesicles / metabolism

Substances

  • Drosophila Proteins
  • Glycoproteins
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • sca protein, Drosophila
  • Green Fluorescent Proteins
  • Cytochalasin D
  • Mitogen-Activated Protein Kinases
  • nmo protein, Drosophila