Increased plasma S-nitrosothiol levels in chronic haemodialysis patients

Nephrol Dial Transplant. 2003 Jan;18(1):153-7. doi: 10.1093/ndt/18.1.153.


Background: An impairment of nitric oxide (NO) bioavailability and/or metabolism may contribute to the excessive incidence of atherosclerotic complications observed in haemodialysis (HD) patients. Recent evidence indicates that NO metabolism involves a family of NO-related molecules that have not yet been explored in such patients. The aim of our study was to determine the plasma levels of S-nitrosothiol and nitrotyrosine in chronic HD patients, and to evaluate potential factors influencing their levels.

Methods: Plasma levels of S-nitrosothiols and nitrotyrosine were determined in 22 non-smoking HD patients and 12 healthy control subjects, together with albumin, homocysteine, haemoglobin, highly sensitive C-reactive protein (hsCRP) and various components of the oxidant-antioxidant system at the plasma and erythrocyte levels.

Results: While plasma nitrosothiol levels were significantly higher in HD patients than in controls (2.25 +/- 1.17 vs 0.45 +/- 0.45 micromol/l, respectively, P < 0.0001), nitrotyrosine levels were not different. HD patients also exhibited a marked deficit of ascorbate and low plasma glutathione peroxidase activity. An inverse relationship was found between plasma S-nitrosothiol and blood haemoglobin in HD patients (P < 0.005). No direct relationship was observed between plasma S-nitrosothiol levels and any of the oxidative stress markers, or hsCRP levels.

Conclusion: This study demonstrates high plasma S-nitrosothiol levels in HD patients, which are partially related to low blood haemoglobin concentrations. The pathophysiological significance of this elevation remains to be elucidated. A possible protective role against nitrosative stress is suggested in presence of normal plasma nitrotyrosine levels in such patients.

Publication types

  • Comparative Study

MeSH terms

  • Biomarkers / blood
  • C-Reactive Protein / metabolism
  • Erythrocytes / metabolism
  • Female
  • Glutathione / blood
  • Glutathione Peroxidase / blood
  • Humans
  • Iron / blood
  • Kidney Diseases / complications
  • Kidney Failure, Chronic / blood
  • Kidney Failure, Chronic / therapy
  • Male
  • Middle Aged
  • Nitric Oxide / metabolism
  • Reference Values
  • Renal Dialysis*
  • S-Nitrosothiols / blood*
  • Transferrin / metabolism
  • Tyrosine / analogs & derivatives*
  • Tyrosine / blood


  • Biomarkers
  • S-Nitrosothiols
  • Transferrin
  • Nitric Oxide
  • 3-nitrotyrosine
  • Tyrosine
  • C-Reactive Protein
  • Iron
  • Glutathione Peroxidase
  • Glutathione