Identification of neuropeptide FF-related peptides in human cerebrospinal fluid by mass spectrometry

FEBS Lett. 2002 Dec 18;532(3):313-8. doi: 10.1016/s0014-5793(02)03686-4.


Several neuropeptide FF (NPFF)-related peptides, known as modulators of the opioid system, have been previously characterized in bovine and rodent brain. Reverse-phase high pressure liquid chromatography (HPLC) fractions of a human with normal pressure hydrocephalus cerebrospinal fluid (CSF), co-migrating with NPFF-related synthetic peptides, were characterized by capillary HPLC coupled on-line to nanospray ion trap tandem mass spectrometry. Two peptides present in the pro-NPFF(A) precursor, NPAF (AGEGLNSQFWSLAAPQRF-NH2) and NPSF (SLAAPQRF-NH2), were identified. The monitoring of NPFF-related peptides in human CSF can be helpful to understand their roles in pain sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cerebrospinal Fluid / metabolism*
  • Chromatography, High Pressure Liquid
  • Humans
  • Mass Spectrometry / methods*
  • Molecular Sequence Data
  • Oligopeptides / cerebrospinal fluid
  • Oligopeptides / chemistry*
  • Peptides / chemistry*
  • Radioimmunoassay
  • Time Factors


  • Oligopeptides
  • Peptides
  • phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide