Diversity and evolution of the envelope gene of dengue virus type 1

Virology. 2002 Nov 10;303(1):110-9. doi: 10.1006/viro.2002.1686.


The genetic diversity and phylogenetic relationships of a collection of strains of dengue virus type 1 (DV-1), isolated from different parts of the world, were investigated. Phylogenetic trees derived from the complete sequence of the E gene of 44 strains suggested the existence of five genetic types defined by a maximum nucleotide divergence within each group of 6%. The 22 strains from America were classified into a single genetic type that included strains associated either with classical dengue or hemorrhagic dengue episodes. Using a maximum likelihood procedure based on a single rate with dated tips model and substitution rates calculated at the third codon position, evolution of the five DV-1 genotypes was shown to conform to a molecular clock. The average rate of evolution was estimated to be approximately 16.2 x 10(-4) substitutions/third codon position site/year. Using this estimate, divergence among the DV-1 genotypes was calculated to have occurred approximately 100 years ago. Very low average value of the ratio of nonsynonymous-to-synonymous nucleotide substitutions, relative to the respective sites (0.046), indicated that the evolution of the E gene of the DV-1 is subject mostly to purifying selection.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dengue / epidemiology
  • Dengue Virus / genetics*
  • Evolution, Molecular
  • Genetic Variation
  • Genotype
  • Global Health
  • Humans
  • Molecular Epidemiology
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / genetics
  • Sequence Analysis
  • Time Factors
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / classification
  • Viral Envelope Proteins / genetics*


  • RNA, Viral
  • Viral Envelope Proteins
  • E protein TH Sman, Dengue virus

Associated data

  • GENBANK/AF425609
  • GENBANK/AF425610
  • GENBANK/AF425611
  • GENBANK/AF425612
  • GENBANK/AF425613
  • GENBANK/AF425614
  • GENBANK/AF425615
  • GENBANK/AF425616
  • GENBANK/AF425617
  • GENBANK/AF425618
  • GENBANK/AF425619
  • GENBANK/AF425620
  • GENBANK/AF425621
  • GENBANK/AF425622
  • GENBANK/AF425623
  • GENBANK/AF425624
  • GENBANK/AF425625
  • GENBANK/AF425626
  • GENBANK/AF425627
  • GENBANK/AF425628
  • GENBANK/AF425629
  • GENBANK/AF425630
  • GENBANK/AF425631
  • GENBANK/AF425632
  • GENBANK/AF425633
  • GENBANK/AF425634
  • GENBANK/AF425635
  • GENBANK/AF425636
  • GENBANK/AF425637
  • GENBANK/AF425638
  • GENBANK/AF425639