Dorsal skin responses to topical application with hydrogen peroxide in Mini and Wistar rats

Exp Toxicol Pathol. 2002 Nov;54(3):239-44. doi: 10.1078/0940-2993-00256.


Mini rats (Jcl: WistarTGN(ARGHGEN) 1Nts) (MRs) are Wistar rat (WR)-derived transgenic rats in which the expression of growth hormone (GH) gene is suppressed under the presence of antisense RNA transgene. In order to evaluate the effects of GH-deficiency on the acute injury by external stimuli, the dorsal skin responses to a single topical application with 20% hydrogen peroxide (HPO), one of the environmental oxidative stressors, were histologically compared between male MRs and WRs of 8 weeks old, whose hair cycle was under the telogen phase. As a result, formation of granulation tissues, reepithelialization and regrowth of hair follicles were delayed in MRs compared with WRs. While hair follicles of MRs of this age are under a long-lasting telogen phase after their 2nd cycle, a new hair cycle started not only in the HPO-applied area but also in the solvent-applied area with a little time lag. These findings suggest that GH-deficiency may influence the skin responses to the external chemical stimuli.

MeSH terms

  • Administration, Topical
  • Animals
  • Growth Hormone / deficiency*
  • Hydrogen Peroxide / toxicity*
  • Male
  • Necrosis
  • Rats
  • Rats, Inbred Strains
  • Rats, Wistar
  • Skin / drug effects*
  • Skin / metabolism
  • Skin / pathology


  • Growth Hormone
  • Hydrogen Peroxide