Preparation of dissociated zebrafish spinal neuron cultures

Methods Cell Sci. 2001;23(4):205-9. doi: 10.1023/a:1016349232389.

Abstract

The method described here explains a simple protocol for how to prepare dissociated Zebrafish spinal neuron cultures. The neurons grow fast in a simple culture medium and at room temperature. Considering the advantages afforded by the optical transparency of the Zebrafish embryo combined with the powerful molecular perturbation techniques available, this technique has potential to further advance molecular analysis of axon growth and guidance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods*
  • Cell Differentiation / physiology
  • Cell Division / drug effects
  • Cell Division / physiology
  • Cell Separation / instrumentation
  • Cell Separation / methods*
  • Culture Media / chemistry
  • Dissection / instrumentation
  • Dissection / methods*
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / embryology*
  • Genetic Testing / trends
  • Growth Cones / drug effects
  • Growth Cones / ultrastructure
  • Neurons / cytology*
  • Spinal Cord / cytology
  • Spinal Cord / embryology*
  • Trypsin
  • Zebrafish / embryology*
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / isolation & purification

Substances

  • Culture Media
  • Zebrafish Proteins
  • Trypsin