Increased expression of alpha-1-antitrypsin, glutathione S-transferase pi and vascular endothelial growth factor in human pancreatic adenocarcinoma

Am J Surg. 2002 Dec;184(6):642-7; discussion 647-8. doi: 10.1016/s0002-9610(02)01105-4.

Abstract

Background: This study was designed to investigate abnormalities in gene expression in ductal adenocarcinoma of the pancreas using cDNA arrays.

Methods: Gene expression in pancreatic ductal adenocarcinoma was compared with normal pancreatic tissue controls. Specimens from 5 patients with pancreatic adenocarcinoma were taken fresh at operation and analyzed using commercially prepared cDNA arrays evaluating approximately 2,000 genes. Immunohistochemical staining was used to confirm protein expression of selected genes.

Results: Alpha-1-antitrypsin (A1AT) and glutathione S-transferase pi (GSTP) were significantly up-regulated in all 5 tumors. Vascular endothelial growth factor (VEGF) was up-regulated in 4 of the 5 patients. Immunohistochemical staining verified the overexpression of each of these genes.

Conclusions: A1AT, GSTP, and VEGF are overexpressed in human pancreatic adenocarcinoma specimens taken fresh at operation. To our knowledge, this is the first study of human pancreatic ductal adenocarcinoma demonstrating the up-regulation of these genes using gene expression arrays.

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Aged
  • Carcinoma, Pancreatic Ductal / genetics
  • Carcinoma, Pancreatic Ductal / pathology
  • Endothelial Growth Factors / genetics*
  • Female
  • Gene Expression
  • Glutathione S-Transferase pi
  • Glutathione Transferase / genetics*
  • Humans
  • Immunohistochemistry / methods
  • Intercellular Signaling Peptides and Proteins / genetics*
  • Isoenzymes / genetics*
  • Lymphokines / genetics*
  • Male
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis / methods
  • Pancreatic Neoplasms / genetics*
  • Pancreatic Neoplasms / pathology
  • Up-Regulation / genetics
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • alpha 1-Antitrypsin / genetics*

Substances

  • Endothelial Growth Factors
  • Intercellular Signaling Peptides and Proteins
  • Isoenzymes
  • Lymphokines
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • alpha 1-Antitrypsin
  • GSTP1 protein, human
  • Glutathione S-Transferase pi
  • Glutathione Transferase