Nitric oxide inhibits cysteine proteinases and alcohol dehydrogenase 2 of Entamoeba histolytica

Parasitol Res. 2003 Jan;89(2):146-9. doi: 10.1007/s00436-002-0716-2. Epub 2002 Aug 15.

Abstract

Nitric oxide is involved in the neutrophil and macrophage killing of the protozoan parasite Entamoeba histolytica. In the present study, we found that cysteine proteinases, significant contributors to amebic virulence and alcohol dehydrogenase 2, an enzyme absolutely required for the survival of the parasite, are both significantly inhibited by S-nitroso-glutathione, a physiological nitric oxide donor, within the concentration range 0.5-2.0 m M.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Dehydrogenase / analysis
  • Alcohol Dehydrogenase / metabolism*
  • Animals
  • Cysteine Endopeptidases / analysis
  • Cysteine Endopeptidases / metabolism*
  • Entamoeba histolytica / drug effects*
  • Entamoeba histolytica / enzymology*
  • Entamoebiasis / drug therapy
  • Host-Parasite Interactions
  • Nitric Oxide / pharmacology
  • Nitric Oxide Donors / pharmacology*
  • S-Nitrosoglutathione / pharmacology*

Substances

  • Nitric Oxide Donors
  • Nitric Oxide
  • S-Nitrosoglutathione
  • Alcohol Dehydrogenase
  • Cysteine Endopeptidases