Study of isolation of fluoroquinolone-resistant Ureaplasma urealyticum and identification of mutant sites

Chin Med J (Engl). 2002 Oct;115(10):1573-5.

Abstract

Objective: To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones.

Methods: Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance-determining region) gyrA, gyrB, parC and parE were amplified by PCR. Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed.

Results: Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine.

Conclusion: These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Anti-Infective Agents / pharmacology*
  • DNA Gyrase / genetics*
  • DNA Topoisomerase IV / genetics*
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Fluoroquinolones
  • Humans
  • Mutation*
  • Polymerase Chain Reaction
  • Ureaplasma urealyticum / drug effects*
  • Ureaplasma urealyticum / genetics
  • Ureaplasma urealyticum / isolation & purification

Substances

  • Anti-Infective Agents
  • Fluoroquinolones
  • DNA Topoisomerase IV
  • DNA Gyrase