Cross-resistance to pyrethroid and organophosphorus insecticides induced by selection with temephos in Aedes aegypti (Diptera: Culicidae) from Cuba

J Med Entomol. 2002 Nov;39(6):882-8. doi: 10.1603/0022-2585-39.6.882.


A sample of Aedes aegypti (L.) from Santiago de Cuba, Cuba, with a high level of temephos resistance (19.58x at the 50% lethal concentration [LC50]), was subjected to temephos selection to evaluate the utility of this organophosphate insecticide for mosquito control. High resistance developed after six generations of selection (200.00x). Little or no cross-resistance was observed to the organophosphates, malathion and fenitrothion, but high cross-resistance was observed for the pyrethroid deltamethrin (337.5x) and the organophosphate fenthion (12.74x). Synergism tests implicated detoxifying esterases in temephos and fenthion resistance and deltamethrin resistance was associated with a cytochrome P450 monooxygenasa. Biochemical tests, polyacrylamide gel electrophoresis (PAGE), and inhibition studies confirmed the presence of elevated esterase activity associated with temephos resistance. Pyrethroid resistance could be associated with a glutathione-S-transferase mechanism but not the esterases. The cross-resistance to deltamethrin from temephos selection could limit the use of both insecticides for Ae. aegypti control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / classification
  • Aedes / drug effects*
  • Animals
  • Cuba
  • Drug Resistance / physiology*
  • Egypt
  • Insecticides / toxicity*
  • Pyrethrins / toxicity*
  • Species Specificity
  • Temefos / toxicity*


  • Insecticides
  • Pyrethrins
  • Temefos