During the early prediabetic period in NOD mice, the pathogenic CD8(+) T-cell population comprises multiple antigenic specificities

Clin Immunol. 2002 Dec;105(3):332-41. doi: 10.1006/clim.2002.5298.


In the NOD mouse model of type 1 diabetes, major histocompatibilitycomplex (MHC) class I-restricted CD8(+) T cells are essential for disease development. However, the extent of diversity of their antigenic specificities during early pathogenesis remains unclear. An insulin-derived peptide was recently identified as the epitope for the NOD-derived diabetogenic T-cell clone G9C8. To explore the possibility that the early pathogenic CD8(+) T-cell population comprises additional antigenic specificities, we employed the T-cell clones AI4 and NY8.3, both of which are pathogenic and represent specificities present in early insulitic lesions. The clones responded to distinct fractions of chromatographically separated class I MHC-bound peptides purified from NOD-derived NIT-1 beta cells, and neither clone recognized the insulin-derived peptide. NIT-1 cells represent an unlimited peptide source that will allow for the future isolation and sequencing of the novel multiple epitopes targeted early in the autoimmune response by pathogenic CD8(+) T cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoimmunity
  • CD8-Positive T-Lymphocytes / immunology*
  • Cell Line, Transformed
  • Clone Cells
  • Diabetes Mellitus, Type 1 / immunology*
  • Epitopes
  • Histocompatibility Antigens Class I / immunology
  • Insulin / immunology
  • Islets of Langerhans / immunology
  • Mice
  • Mice, Inbred NOD
  • Mice, Transgenic
  • Prediabetic State / immunology*
  • T-Lymphocytes, Cytotoxic


  • Epitopes
  • Histocompatibility Antigens Class I
  • Insulin