A polysaccharide, extract from Grifola frondosa, induces Th-1 dominant responses in carcinoma-bearing BALB/c mice

Jpn J Pharmacol. 2002 Dec;90(4):357-60. doi: 10.1254/jjp.90.357.


A polysaccharide, designated as the D-fraction, extracted from maitake (Grifola frondosa), was reported to have anti-tumor effects by activating macrophages and T cells in C3H/HeN mice in which a Th-1 dominant response was established. In this study using BALB/c mice in which a Th-2 response is genetically dominant, D-fraction reduced the expression of Th-2 cytokine interleukin (IL)-4 but markedly increased the expression of Th-1 cytokine interferon (IFN)-gamma in CD4(+) T cells and also increased IL-12p70 production as well as IFN-gamma production by antigen-presenting cells (APCs), suggesting that D-fraction promotes the differentiation into Th-1 cells of CD4(+) T cells through enhancement of IL-12p70 production by APCs.

MeSH terms

  • Agaricales / chemistry*
  • Animals
  • Antigen-Presenting Cells / drug effects
  • Antigen-Presenting Cells / metabolism
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • CD4-Positive T-Lymphocytes / drug effects
  • CD4-Positive T-Lymphocytes / metabolism
  • CD4-Positive T-Lymphocytes / pathology
  • Cell Differentiation / drug effects
  • Female
  • Lymphocyte Activation
  • Macrophages / drug effects
  • Macrophages / pathology
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Transplantation
  • Neoplasms, Experimental / drug therapy*
  • Neoplasms, Experimental / immunology
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Th1 Cells / drug effects*
  • Th1 Cells / metabolism
  • Th1 Cells / pathology
  • Time Factors


  • Antineoplastic Agents
  • Polysaccharides