Multispectral imaging fluorescence microscopy for living cells

Cell Struct Funct. 2002 Oct;27(5):367-74. doi: 10.1247/csf.27.367.


Multispectral imaging technologies have been widely used in fields of astronomy and remote sensing. Interdisciplinary approaches developed in, for example, the National Aeronautics and Space Administration (NASA, USA), the Jet Propulsion Laboratory (JPL, USA), or the Communications Research Laboratory (CRL, Japan) have extended the application areas of these technologies from planetary systems to cellular systems. Here we overview multispectral imaging systems that have been devised for microscope applications. We introduce these systems with particular interest in live cell imaging. Finally we demonstrate examples of spectral imaging of living cells using commercially available systems with no need for user engineering.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Eukaryotic Cells / cytology*
  • Eukaryotic Cells / metabolism
  • Fluorescence Recovery After Photobleaching
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes
  • Humans
  • Microscopy, Confocal
  • Microscopy, Fluorescence*


  • Fluorescent Dyes