The RNA polymerase II CTD kinase CTDK-I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p

Mol Cell. 2002 Dec;10(6):1429-39. doi: 10.1016/s1097-2765(02)00731-1.

Abstract

There are several kinases in Saccharomyces cerevisiae that phosphorylate the CTD of RNA polymerase II, but specific and distinct functions of the phospho-CTDs generated by the different kinases are not well understood. A genetic screen for suppressors of loss of yeast CTD kinase I (CTDK-I) function (by deletion of the catalytic subunit gene CTK1) identified PTI1, a potential 3' cleavage/polyadenylation factor. Genetic and physical interactions connect Pti1p to components of CF IA and CF II/CPF, and mutations of PTI1 or CTK1 affect 3' cleavage site choice and transcript abundance of particular genes. Therefore, one important function of the CTDK-I-generated phospho-CTD appears to be the coupling of transcription to 3' processing of pre-mRNAs by a Pti1p-containing complex.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Genes, Essential
  • Genes, Fungal
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Poly A / metabolism
  • Protein Kinases / metabolism*
  • Protein Subunits / chemistry
  • Protein Subunits / genetics
  • Protein Subunits / metabolism
  • Protein-Serine-Threonine Kinases / metabolism*
  • RNA Polymerase II / chemistry
  • RNA Polymerase II / genetics*
  • RNA Polymerase II / metabolism*
  • RNA Precursors / metabolism*
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Substrate Specificity
  • Transcription, Genetic
  • mRNA Cleavage and Polyadenylation Factors

Substances

  • PTI1 protein, S cerevisiae
  • Peptide Fragments
  • Protein Subunits
  • RNA Precursors
  • Saccharomyces cerevisiae Proteins
  • mRNA Cleavage and Polyadenylation Factors
  • Poly A
  • Protein Kinases
  • carboxy-terminal domain kinase
  • Protein-Serine-Threonine Kinases
  • RNA Polymerase II