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, 10 (6), 1467-77

Dynamics of Enhancer-Promoter Communication During Differentiation-Induced Gene Activation

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Dynamics of Enhancer-Promoter Communication During Differentiation-Induced Gene Activation

Pantelis Hatzis et al. Mol Cell.

Abstract

We analyzed the order of recruitment of factors to the HNF-4alpha regulatory regions upon the initial activation of the gene during enterocyte differentiation. An initially independent assembly of regulatory complexes at the proximal promoter and the upstream enhancer regions was followed by the tracking of the entire DNA-protein complex formed on the enhancer along the intervening DNA until it encountered the proximal promoter. This movement correlated with a unidirectional spreading of histone hyperacetylation. Transcription initiation coincided with the formation of a stable enhancer-promoter complex and remodeling of the nucleosome situated at the transcription start site. The results provide experimental evidence for the involvement of a dynamic process culminating in enhancer-promoter communication during long-distance gene activation.

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