Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 300 (2), 472-6

Resistin Is Expressed in Human Macrophages and Directly Regulated by PPAR Gamma Activators

Affiliations

Resistin Is Expressed in Human Macrophages and Directly Regulated by PPAR Gamma Activators

Lisa Patel et al. Biochem Biophys Res Commun.

Abstract

Resistin is a cysteine-rich protein postulated to be a molecular link between obesity and type 2 diabetes. The aim of this study was to investigate the role of PPAR gamma in the regulation of resistin expression in human primary macrophages. Fluorescent real-time PCR (Taqman) analysis of resistin expression across a range of human tissues showed that resistin is highly expressed in bone marrow compared to other tissues. Taqman analysis and Western blotting showed that rosiglitazone decreased resistin expression at both the mRNA and protein levels in human primary monocyte-derived macrophages in vitro. Resistin expression was reduced by up to 80% after exposure to 100 nM rosiglitazone for 96 h. Bioinformatics analysis of the genomic sequence upstream of the resistin coding sequence identified several putative PPAR response elements of which one was shown to bind PPAR gamma using electrophoretic mobility shift assays. Our data support a direct role for PPAR gamma in the regulation of resistin expression.

Similar articles

See all similar articles

Cited by 218 PubMed Central articles

See all "Cited by" articles

MeSH terms

LinkOut - more resources

Feedback