Liquid chromatography method for the analysis of adenosine compounds

J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jan 25;784(1):189-93. doi: 10.1016/s1570-0232(02)00752-3.

Abstract

A newly available chromatography column packing material that employs hybrid particle technology was used to improve the analysis of adenosine compounds. Using a TBAS buffer/acetonitrile gradient this material permits separation of etheno-adenosine compounds in less than 4 min with excellent resolution and sensitivity (50 fmol). Variability of compound quantification is small (coefficients of variation 0.23+/-0.14% for 50 pmol and 1.70+/-0.53% for 0.5 pmol). The new method is well suited for the analysis of adenosine compounds in small biological samples and permits a high sample throughput in autosampler setups with high precision and reproducibility.

MeSH terms

  • Adenosine / analysis*
  • Chromatography, High Pressure Liquid / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence

Substances

  • Adenosine