TGF-beta 1 and IL-10 modulate IL-1 beta-induced membrane and soluble ICAM-1 in human myoblasts

J Neuroimmunol. 2003 Jan;134(1-2):151-7. doi: 10.1016/s0165-5728(02)00399-5.

Abstract

We have previously shown that interleukin (IL)-1 beta and other inflammatory cytokines are able to induce the expression of membrane and soluble intercellular adhesion molecule (ICAM)-1 on human myoblasts. In this paper we found that IL-10 and transforming growth factor (TGF)-beta 1 are able to prevent IL-1 beta-induced membrane and soluble ICAM-1 protein expression on human myoblasts, with different time courses. The effect of both cytokines is associated to a reduction in ICAM-1 mRNA. Our findings suggest that IL-10 and TGF-beta 1 are able to influence the inflammatory process in muscle tissue at least in part by means of control of membrane and soluble ICAM-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / drug effects
  • Cell Membrane / immunology
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Down-Regulation / drug effects
  • Down-Regulation / immunology
  • Graft Rejection / immunology*
  • Graft Rejection / metabolism
  • Graft Rejection / physiopathology
  • Humans
  • Intercellular Adhesion Molecule-1 / genetics
  • Intercellular Adhesion Molecule-1 / immunology*
  • Intercellular Adhesion Molecule-1 / metabolism
  • Interleukin-1 / immunology*
  • Interleukin-1 / metabolism
  • Interleukin-10 / immunology
  • Interleukin-10 / metabolism*
  • Interleukin-10 / pharmacology
  • Muscle Cells / drug effects
  • Muscle Cells / immunology
  • Muscle Cells / metabolism
  • Muscle, Skeletal / immunology
  • Muscle, Skeletal / metabolism
  • Muscle, Skeletal / physiopathology
  • Myoblasts, Skeletal / drug effects
  • Myoblasts, Skeletal / immunology*
  • Myoblasts, Skeletal / metabolism
  • Myositis / immunology*
  • Myositis / metabolism
  • Myositis / physiopathology
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism
  • Reaction Time / drug effects
  • Reaction Time / immunology
  • Transforming Growth Factor beta / immunology
  • Transforming Growth Factor beta / metabolism*
  • Transforming Growth Factor beta / pharmacology
  • Transforming Growth Factor beta1

Substances

  • Interleukin-1
  • RNA, Messenger
  • TGFB1 protein, human
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta1
  • Intercellular Adhesion Molecule-1
  • Interleukin-10

Grants and funding