Interferon-gamma enhances the pulmonary CXC chemokine response to intratracheal lipopolysaccharide challenge

J Infect Dis. 2003 Jan 1;187(1):62-9. doi: 10.1086/346027. Epub 2002 Dec 13.


CXC chemokines are major chemoattractants for pulmonary polymorphonuclear leukocyte (PMNL) recruitment. To study the effects of interferon (IFN)-gamma on the pulmonary chemokine response to lipopolysaccharide (LPS) challenge, rats were treated with intratracheal IFN-gamma (1x10(5) U/rat) 24 h before an intratracheal LPS (100 microg/rat) challenge. Intratracheal LPS caused significant increases in both cytokine-induced neutrophil chemoattractant (CINC) and macrophage inflammatory protein-2 in bronchoalveolar lavage (BAL) fluid and pulmonary PMNL recruitment. IFN-gamma enhanced these responses. IFN-gamma also increased LPS-induced tumor necrosis factor (TNF)-alpha in BAL fluid. LPS-induced TNF-alpha and CINC mRNA expression in alveolar macrophages was increased by IFN-gamma. CD11b/c and CD18 expression on circulating PMNLs was not affected by IFN-gamma, nor was the chemotaxis of these cells. IFN-gamma increases the pulmonary CXC chemokine response, which may serve as one mechanism underlying enhanced PMNL delivery into the lung.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CD18 Antigens / biosynthesis
  • Chemokine CXCL2
  • Chemokines / biosynthesis*
  • Chemokines / genetics
  • Chemokines, CXC*
  • Chemotactic Factors / biosynthesis*
  • Chemotactic Factors / genetics
  • Injections, Spinal
  • Intercellular Signaling Peptides and Proteins / biosynthesis*
  • Intercellular Signaling Peptides and Proteins / genetics
  • Interferon-gamma / pharmacology*
  • Lipopolysaccharides / administration & dosage
  • Lipopolysaccharides / pharmacology*
  • Lung / immunology*
  • Male
  • Monokines / biosynthesis*
  • Neutrophils / physiology
  • RNA, Messenger
  • Rats
  • Rats, Sprague-Dawley
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Tumor Necrosis Factor-alpha / genetics


  • CD18 Antigens
  • Chemokine CXCL2
  • Chemokines
  • Chemokines, CXC
  • Chemotactic Factors
  • Cxcl2 protein, rat
  • Intercellular Signaling Peptides and Proteins
  • Lipopolysaccharides
  • Monokines
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma