In summary, isolation of GnRH receptor cDNA, its gene, and identification of regulatory elements in the flanking region of the gene have added to our knowledge regarding the tissue-specific expression of the GnRH receptor gene, and the mechanisms that mediate and influence its transcriptional regulation. However, the interactions of the different regulatory factors (nuclear factors) and the effects of these interactions on the regulation of the GnRH receptor gene remain unclear. Due to existence of multiple promoters and transcriptional start sites in human GnRH receptor gene and the lack of a human gonadotrope cell line, the precise promoter and transcriptional start sites in human pituitary, extra-pituitary tissues and tumors have not yet been identified.