Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost

Int J Syst Evol Microbiol. 2002 Nov;52(Pt 6):2251-2255. doi: 10.1099/00207713-52-6-2251.


A thermophilic, spore-forming rod isolated from hay compost in Korea was subjected to a taxonomic study. The micro-organism, designated strain SK-1(T), was identified as being aerobic, Gram-positive, motile and rod-shaped. Growth of the isolate was observed at 45-70 degrees C (optimum 60 degrees C) and pH 6.0-9.0 (optimum pH 7.5). The G+C content of the genomic DNA was 43.9 mol%. Chemotaxonomic characteristics of the isolate included the presence of mesodiaminopimelic acid in the cell wall and iso-C15:0 and iso-C17:0 as the major cellular fatty acids. The predominant isoprenoid quinone was MK-7. The chemotaxonomic characteristics of strain SK-1(T) were the same as those of the genus Geobacillus. Phylogenetic analysis based on 16S rDNA sequences showed that strain SK-1(T) is most closely related to Geobacillus thermoglucosidasius. However, the phenotypic properties of strain SK-1(T) were clearly different from those of G. thermoglucosidasius. The level of DNA-DNA relatedness between strain SK-1(T) and the type strain of G. thermoglucosidasius was 27%. On the basis of the phenotypic traits and molecular systematic data, strain SK-1(T) represents a novel species within the genus Geobacillus, for which the name Geobacillus toebii sp. nov. is proposed. The type strain is strain SK-1(T) (= KCTC 0306BP(T) - DSM 14590(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Gram-Positive Endospore-Forming Rods / classification*
  • Gram-Positive Endospore-Forming Rods / genetics
  • Gram-Positive Endospore-Forming Rods / isolation & purification
  • Gram-Positive Endospore-Forming Rods / metabolism
  • Hot Temperature
  • Korea
  • Molecular Sequence Data
  • Phenotype
  • Phylogeny
  • Poaceae / microbiology
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Terminology as Topic


  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Bacterial
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AF326278