Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP

Med Vet Entomol. 2002 Dec;16(4):461-4. doi: 10.1046/j.1365-2915.2002.00393.x.


For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Africa
  • Aging
  • Animals
  • Anopheles / classification*
  • Anopheles / genetics*
  • Base Sequence
  • Genes, Insect*
  • Larva / classification
  • Larva / genetics
  • Polymerase Chain Reaction*
  • Polymorphism, Restriction Fragment Length*
  • Species Specificity