Abstract
The adaptor molecule, linker for activation of T cells (LAT), is essential in T cell activation and development; a similar molecule in B cells has not yet been identified. Here, we report the identification of a new adaptor protein, linker for activation of B cells (LAB). Like LAT, LAB was localized to lipid rafts. Upon activation via the B cell receptor (BCR), LAB was phosphorylated and interacted with the adaptor protein Grb2. Decreased LAB expression led to a reduction in BCR-mediated calcium flux and Erk activation. LAB rescued thymocyte development but not normal T cell activation in LAT(-/-) mice. Our data suggest that LAB links BCR engagement to downstream signaling pathways.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Adaptor Proteins, Vesicular Transport / genetics
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Adaptor Proteins, Vesicular Transport / immunology*
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Adaptor Proteins, Vesicular Transport / metabolism
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Amino Acid Sequence
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Animals
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B-Lymphocytes / immunology*
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Carrier Proteins / genetics
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Carrier Proteins / immunology
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Cloning, Molecular
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Humans
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Lymphocyte Activation
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Membrane Proteins*
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Molecular Sequence Data
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Phosphoproteins / deficiency
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Phosphoproteins / genetics
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Phosphoproteins / immunology
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Phosphorylation
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Proteins*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Signal Transduction
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Tissue Distribution
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Tyrosine / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport
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Carrier Proteins
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LAB protein, mouse
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LAT protein, human
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LAT2 protein, human
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LAT2 protein, mouse
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Lat protein, mouse
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Membrane Proteins
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Phosphoproteins
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Proteins
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RNA, Messenger
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Tyrosine
Associated data
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GENBANK/AY190023
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GENBANK/AY190024