The orphan nuclear receptor HNF4alpha determines PXR- and CAR-mediated xenobiotic induction of CYP3A4

Nat Med. 2003 Feb;9(2):220-4. doi: 10.1038/nm815. Epub 2003 Jan 6.

Abstract

The drug metabolizing enzyme cytochrome P450 3A4 (CYP3A4) is thought to be involved in the metabolism of nearly 50% of all the drugs currently prescribed. Alteration in the activity or expression of this enzyme seems to be a key predictor of drug responsiveness and toxicity. Currently available studies indicate that the ligand-activated nuclear receptors pregnane X receptor (PXR; NR1I2) and constitutive androstane receptor (CAR; NR1I3) regulate CYP3A4 expression. However, in cell-based reporter assays, CYP3A4 promoter activity was most pronounced in liver-derived cells and minimal or modest in non-hepatic cells, indicating that a liver-specific factor is required for physiological transcriptional response. Here we show that the orphan nuclear receptor hepatocyte nuclear factor-4alpha (HNF4alpha; HNF4A) is critically involved in the PXR- and CAR-mediated transcriptional activation of CYP3A4. We identified a specific cis-acting element in the CYP3A4 gene enhancer that confers HNF4alpha binding and thereby permits PXR- and CAR-mediated gene activation. Fetal mice with conditional deletion of Hnf4alpha had reduced or absent expression of CYP3A. Furthermore, adult mice with conditional hepatic deletion of Hnf4alpha had reduced basal and inducible expression of CYP3A. These data identify HNF4alpha as an important regulator of coordinate nuclear-receptor-mediated response to xenobiotics.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Cytochrome P-450 CYP3A
  • Cytochrome P-450 Enzyme System / biosynthesis*
  • Cytochrome P-450 Enzyme System / genetics
  • DNA Primers
  • DNA-Binding Proteins*
  • Electrophoretic Mobility Shift Assay
  • Enhancer Elements, Genetic
  • Enzyme Induction
  • Hepatocyte Nuclear Factor 4
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Phosphoproteins / genetics
  • Phosphoproteins / physiology*
  • Pregnane X Receptor
  • Receptors, Cytoplasmic and Nuclear / physiology*
  • Receptors, Steroid / physiology*
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Tumor Cells, Cultured
  • Xenobiotics / pharmacology*

Substances

  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • DNA Primers
  • DNA-Binding Proteins
  • HNF4A protein, human
  • Hepatocyte Nuclear Factor 4
  • Hnf4a protein, mouse
  • MLX protein, human
  • NR1I2 protein, human
  • Nr1i2 protein, mouse
  • Phosphoproteins
  • Pregnane X Receptor
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Steroid
  • Tcfl4 protein, mouse
  • Transcription Factors
  • Xenobiotics
  • constitutive androstane receptor
  • Cytochrome P-450 Enzyme System
  • CYP3A protein, human
  • Cytochrome P-450 CYP3A
  • CYP3A4 protein, human