Objective: To elucidate the mechanism of anti-inflammatory action of Huanglian Jiedu Decoction (HJD).
Methods: An acute inflammatory air-pouch was established in mice by s.c. injecting air and 1% carrageenan. A mouse endotoxeamia induced by lipopolysaccharide was also used. Splenocyte proliferation was quantified with 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetery. NO2- concentration was examined by a Griess reagent and interleukin-1 activity was evaluated in thymocyte proliferation.
Results: HJD significantly inhibited the leukocyte infiltration and PGE2 production in the mouse air-pouch model. In the in vitro assay, HJD significantly inhibited concanavalin A(Con A)-induced transformation of spleen cells isolated from the endotoxeamia mice, but did not influence that from the normal mice. HJD did not affact Con A-induced interleukin-2 production in splenocytes in either normal or endotoxeamia mice. HJD remarkably reduced interleukin-1 and NO production in peritoneal macrophages elicited by lipopol ysaccharide.
Conclusion: HJD displays an anti-inflammatory effect mainly through inhibiting the production of inflammatory cytokines including IL-1, NO and PGE2.