Abstract
Rapid, sensitive, and sequence-specific DNA detection can be achieved in one step using an engineered intrasterically regulated enzyme. The semi-synthetic inhibitor-DNA-enzyme (IDE) construct (left) rests in the inactive state but upon exposure to a complementary DNA sequence undergoes a DNA hybridization-triggered allosteric enzyme activation (right). The ensuing rapid substrate turnover provides the built-in signal amplification mechanism for detecting approximately 10 fmol DNA in less than 3 min under physiological conditions.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacillus cereus / enzymology*
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Bacillus cereus / genetics
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DNA / analysis*
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DNA, Single-Stranded / chemistry
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Kinetics
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Metalloendopeptidases / chemistry*
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Metalloendopeptidases / genetics
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Metalloendopeptidases / metabolism
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Oligopeptides / chemistry
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Oligopeptides / metabolism
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Protease Inhibitors / chemistry*
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Signal Processing, Computer-Assisted
Substances
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DNA, Single-Stranded
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Oligopeptides
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Protease Inhibitors
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DNA
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Metalloendopeptidases