Characterization of human NOV in biological fluids: an enzyme immunoassay for the quantification of human NOV in sera from patients with diseases of the adrenal gland and of the nervous system

J Clin Endocrinol Metab. 2003 Jan;88(1):327-36. doi: 10.1210/jc.2002-020304.


Immunochromatography has shown that human NOV (NOVH), a member of the CCN (CTGF/CYR61/NOV) family, forms a physiological complex with fibulin-1 in blood. We developed an enzyme immunoassay specific for NOVH and showed for the first time that the concentration of NOVH differs in each of these biological fluids. The normal concentration of NOVH circulating in the blood is 350-400 ng/ml, but this concentration varies with age. By using sera from patients with adrenal gland diseases we found that in vivo ACTH or glucocorticoids are not responsible for the high concentration of NOVH in this endocrine gland. However, the NOVH concentration was significantly modified in malignant adrenocortical tumors, but not in benign adrenocortical tumors. The concentration of NOVH was significantly decreased in patients suffering from astrocytomas or multiple sclerosis, two diseases of the nervous system. Thus, NOVH is a potentially useful marker for the diagnosis of these diseases.

MeSH terms

  • Adolescent
  • Adrenal Gland Diseases / blood*
  • Adult
  • Aged
  • Body Fluids / metabolism*
  • Calcium-Binding Proteins / blood
  • Calcium-Binding Proteins / isolation & purification
  • Connective Tissue Growth Factor
  • Female
  • Humans
  • Immediate-Early Proteins / blood
  • Immediate-Early Proteins / isolation & purification
  • Immediate-Early Proteins / metabolism*
  • Immunoenzyme Techniques / methods*
  • Intercellular Signaling Peptides and Proteins / blood
  • Intercellular Signaling Peptides and Proteins / isolation & purification
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Male
  • Middle Aged
  • Nephroblastoma Overexpressed Protein
  • Nervous System Diseases / blood*
  • Sensitivity and Specificity
  • Tumor Cells, Cultured


  • CCN2 protein, human
  • CCN3 protein, human
  • Calcium-Binding Proteins
  • Immediate-Early Proteins
  • Intercellular Signaling Peptides and Proteins
  • Nephroblastoma Overexpressed Protein
  • fibulin
  • Connective Tissue Growth Factor