The aim of this research is two-fold. First, it examined individuals' attitudes towards homosexuals in Singapore. Second, it also investigated whether gender differences in attitudes towards homosexuals exist. Respondents comprised 365 students and data were collected through the use of questionnaires. Results generally suggested that respondents harboured negative attitudes towards homosexuals. The prevalence of such ambivalent attitudes seemed to suggest that respondents were still generally quite conservative in their attitudes towards gender roles and homosexuality. Our findings also revealed that, generally, women reported that they were more comfortable in working closely with male homosexuals while the reverse was true for men. Implications of our findings were discussed.