No increase in lifespan in Caenorhabditis elegans upon treatment with the superoxide dismutase mimetic EUK-8

Free Radic Biol Med. 2003 Jan 15;34(2):277-82. doi: 10.1016/s0891-5849(02)01290-x.


The superoxide dismutase mimetic EUK-8 has been reported to extend lifespan in the nematode Caenorhabditis elegans. However, in five trials administering EUK-8 in liquid culture with E. coli, and two trials using defined liquid medium, we observed no increase in C. elegans lifespan. Instead we saw a dose-dependent reduction of lifespan and fertility. We conclude that extension of C. elegans lifespan by EUK-8 may only occur under very particular culture conditions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Caenorhabditis elegans / drug effects*
  • Caenorhabditis elegans / physiology
  • Dose-Response Relationship, Drug
  • Ethylenediamines / pharmacology*
  • Fertility / drug effects
  • Longevity / drug effects*
  • Molecular Mimicry
  • Organometallic Compounds / pharmacology*
  • Superoxide Dismutase*
  • Time Factors


  • Ethylenediamines
  • Organometallic Compounds
  • N,N'-bis(salicylideneamino)ethane-manganese(II)
  • Superoxide Dismutase