Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies

Clin Diagn Lab Immunol. 2003 Jan;10(1):19-21. doi: 10.1128/cdli.10.1.19-21.2003.


We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4 degrees C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Antibodies, Viral / blood*
  • Cryopreservation
  • Enzyme-Linked Immunosorbent Assay
  • Freezing
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Measles / diagnosis
  • Measles / immunology
  • Mumps / diagnosis
  • Mumps / immunology
  • RNA Virus Infections / diagnosis*
  • RNA Virus Infections / immunology
  • Reproducibility of Results
  • Rubella / diagnosis
  • Rubella / immunology


  • Antibodies, Viral
  • Immunoglobulin G
  • Immunoglobulin M