Comparison of serological assays for detection of Chlamydia trachomatis antibodies in different groups of obstetrical and gynecological patients

Clin Diagn Lab Immunol. 2003 Jan;10(1):174-6. doi: 10.1128/cdli.10.1.174-176.2003.


New serological enzyme immunoassays (EIAs) were compared with microimmunofluorescence (MIF) as a "gold standard" to detect Chlamydia trachomatis antibodies in different groups of obstetrical, gynecological, and subfertile patients. There were no significant differences in seroprevalence rates, except for the group of C. trachomatis-positive patients (P < 0.01). Test characteristics were calculated for Chlamydia-EIA (Biologische Analysensystem GmbH, Lich, Germany) and pELISA (Medac, Wedel, Germany). pELISA seems to be a good alternative to MIF. It has high specificity and is easier to perform.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Antibodies, Bacterial / blood*
  • Chlamydia Infections / diagnosis*
  • Chlamydia trachomatis / immunology*
  • Female
  • Fertility
  • Humans
  • Immunoassay / methods
  • Immunoassay / standards
  • Infertility / microbiology*
  • Pregnancy
  • Prevalence
  • Sensitivity and Specificity
  • Serologic Tests / methods*
  • Serologic Tests / standards


  • Antibodies, Bacterial