Identification of a novel human DDX40gene, a new member of the DEAH-box protein family

J Hum Genet. 2002;47(12):681-3. doi: 10.1007/s100380200104.


The DExH/D-box superfamily of RNA helicases seems to play key roles during RNA metabolism, such as pre-mRNA splicing, ribosome biogenesis, and others. We have cloned a new gene of the DEAH-box protein subgroup, designated DDX40 (DEAD/H-box polypeptide 40 gene). DDX40 contains 3656 nucleotides and codes for a putative 779-amino-acid protein. Sequence analysis of the cDNA product revealed that it contained a DEAH (Asp-Glu-Ala-His) sequence motif and other conserved motifs. The DDX40 protein shared 53% and 43% amino acid identity with human DDX8 and yeast Drh1, respectively, in the conserved region. Northern blot analysis showed that DDX40 was expressed ubiquitously in the eight tissues examined, implying a general physiological function of the protein. We speculate that, like other members of the DExH/D-box superfamily, DDX40 may play roles in pre-mRNA splicing, ribosome biogenesis and other RNA processing functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Northern
  • Chromosomes, Human, Pair 17*
  • Cloning, Molecular
  • DEAD-box RNA Helicases
  • Humans
  • Molecular Sequence Data
  • RNA Helicases / chemistry
  • RNA Helicases / genetics*
  • RNA Helicases / isolation & purification
  • RNA Helicases / metabolism
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tissue Distribution


  • RNA, Messenger
  • DHX40 protein, human
  • DEAD-box RNA Helicases
  • RNA Helicases

Associated data

  • GENBANK/AF461690