Physical and Functional Interactions Among Basic Chromosome Organizational Features Govern Early Steps of Meiotic Chiasma Formation

Cell. 2002 Dec 13;111(6):791-802. doi: 10.1016/s0092-8674(02)01167-4.

Abstract

Analysis of meiotic recombination by functional genomic approaches reveals prominent spatial and functional interactions among diverse organizational determinants. Recombination occurs between chromatin loop sequences; however, these sequences are spatially tethered to underlying chromosome axes via their recombinosomes. Meiotic chromosomal protein, Red1, localizes to chromosome axes; however, Red1 loading is modulated by R/G-bands isochores and thus by bulk chromatin state. Recombination is also modulated by isochore determinants: R-bands differentially favor double-strand break (DSB) formation but disfavor subsequent loading of meiotic RecA homolog, Dmc1. Red1 promotes DSB formation in both R- and G-bands and then promotes Dmc1 loading, specifically counteracting disfavoring R-band effects. These complexities are discussed in the context of chiasma formation as a series of coordinated local changes at the DNA and chromosome-axis levels.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Cycle Proteins*
  • Chromatin / ultrastructure
  • Chromosomes / ultrastructure*
  • DNA / ultrastructure
  • DNA Damage
  • DNA-Binding Proteins / metabolism
  • Epitopes
  • Fungal Proteins
  • Genes, Plant
  • Meiosis*
  • Mitosis
  • Models, Biological
  • Nucleic Acid Hybridization
  • Precipitin Tests
  • Protein Structure, Tertiary
  • Recombination, Genetic*
  • Saccharomyces cerevisiae Proteins / metabolism

Substances

  • Cell Cycle Proteins
  • Chromatin
  • DMC1 protein, S cerevisiae
  • DNA-Binding Proteins
  • Epitopes
  • Fungal Proteins
  • RED1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • DNA