Study of anti-inflammatory activity of Tibetan mushroom, a symbiotic culture of bacteria and fungi encapsulated into a polysaccharide matrix

Pharmacol Res. 2003 Jan;47(1):49-52. doi: 10.1016/s1043-6618(02)00240-2.


Tibetan mushroom (TM) is a fermented beverage composed by a dozen of bacteria and yeasts living together into polysaccharide grains secreted by them. TM is similar to kefir, a probiotic beverage originated in the Caucasian mountains exhibiting some anti-bacterial, anti-mycotic, anti-neoplastic and immunomodulatory effects. Aiming to evaluate a plausible anti-inflammatory property of TM we conducted cotton-induced granuloma and paw edema assays in rats, the latter using carrageenin, dextran and histamine as stimuli. TM samples were thawed and continuously cultured during 15 days into molasses solutions (50 g/l). The experiments used TM suspensions after 24h fermentation and TM grains mechanically disintegrated. The results showed a significant inhibition on the formation of granuloma tissue for the test group as compared to the negative control group. TM suspensions presented an inhibition of 43% for the inflammatory process. Rat paw edema also showed significant decreases with the mediators. The edema induced by carrageenin was inhibited 62% at the 3rd hour. The edema dextran-induced was completely inhibited at 1h and antagonized the histamine edema 52% at 1h.

MeSH terms

  • Agaricales*
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
  • Anti-Inflammatory Agents, Non-Steroidal / therapeutic use*
  • Bacteria / growth & development*
  • Bacteria / isolation & purification
  • Beverages / microbiology
  • Drug Compounding / methods
  • Edema / drug therapy
  • Female
  • Fermentation / physiology
  • Fungi / growth & development*
  • Fungi / isolation & purification
  • Male
  • Medicine, Tibetan Traditional*
  • Polysaccharides / isolation & purification
  • Polysaccharides / therapeutic use*
  • Rats
  • Rats, Wistar
  • Symbiosis / physiology*


  • Anti-Inflammatory Agents, Non-Steroidal
  • Polysaccharides