Effect of genotype on steady-state CYP1A1 gene expression in human peripheral lymphocytes

Biochem Pharmacol. 2003 Feb 1;65(3):441-5. doi: 10.1016/s0006-2952(02)01546-0.

Abstract

We have analyzed the steady-state levels of cytochrome P-450 1A1 (CYP1A1) mRNA in peripheral blood lymphocytes of 177 individuals with various CYP1A1 genotypes using a quantitative reverse transcriptase-polymerase chain reaction technique that makes use of a homologous internal standard for accurate quantitation. We found no effects of ethnicity, age, or smoking status on CYP1A1 gene expression in this population. We did see a significant 2-fold increase in the mean level of CYP1A1 mRNA in women compared with men for both Caucasians and African Americans. We observed no effect of the African American-specific polymorphism (CYP1A1(*)3) on expression of the gene. However, we found a significant 3-fold decrease in expression associated with the homozygous MspI restriction fragment length polymorphism (CYP1A1(*)2A/(*)2A).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • African Americans
  • Cytochrome P-450 CYP1A1 / biosynthesis*
  • Cytochrome P-450 CYP1A1 / genetics
  • Gene Expression
  • Genotype
  • Humans
  • In Vitro Techniques
  • Lymphocytes / enzymology
  • Lymphocytes / metabolism*
  • Polymorphism, Genetic
  • RNA Stability
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • RNA, Messenger
  • Cytochrome P-450 CYP1A1